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GluT1 antibody [GLUT1/2476]

Anti-GluT1 antibody [GLUT1/2476] used in IHC (Paraffin sections) (IHC-P). GTX17991
Anti-GluT1 antibody [GLUT1/2476] used in Flow cytometry (FACS). GTX17991
Anti-GluT1 antibody [GLUT1/2476] used in Western Blot (WB). GTX17991
Anti-GluT1 antibody [GLUT1/2476] used in IHC (Paraffin sections) (IHC-P). GTX17991
Anti-GluT1 antibody [GLUT1/2476] used in Protein Array (Protein Array). GTX17991
Anti-GluT1 antibody [GLUT1/2476] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17991

Cat. No. GTX17991

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GLUT1/2476

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all GluT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
ELISA 1-2μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CSE , DYT17 , DYT18 , DYT9 , EIG12 , GLUT , GLUT1 , GLUT1DS , HTLVR , PED , SDCHCN , SLC2A1 , solute carrier family 2 member 1 , Glucose Transporter GLUT1

Cellular Localization

Cell membrane,Melanosome

Background

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

Database

Research Area

DATA IMAGES

Anti-GluT1 antibody [GLUT1/2476] used in IHC (Paraffin sections) (IHC-P). GTX17991

GTX17991 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX17991 GluT1 antibody [GLUT1/2476].

Anti-GluT1 antibody [GLUT1/2476] used in Flow cytometry (FACS). GTX17991

GTX17991 FACS Image

FACS analysis of K562 cells using GTX17991 GluT1 antibody [GLUT1/2476].
Blue : Primary antibody
Red : Isotype control

Anti-GluT1 antibody [GLUT1/2476] used in Western Blot (WB). GTX17991

GTX17991 WB Image

WB analysis of A431 cell lysate using GTX17991 GluT1 antibody [GLUT1/2476].

Anti-GluT1 antibody [GLUT1/2476] used in IHC (Paraffin sections) (IHC-P). GTX17991

GTX17991 IHC-P Image

IHC-P analysis of human bladder tissue using GTX17991 GluT1 antibody [GLUT1/2476].

Anti-GluT1 antibody [GLUT1/2476] used in Protein Array (Protein Array). GTX17991

GTX17991 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-GluT1 antibody [GLUT1/2476] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17991

GTX17991 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX17991 GluT1 antibody [GLUT1/2476].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529